Program Project: Host Virus Interactions in HCoV-SARS Infections

References

  1. Pewe L, Zhou H, Netland, J, Tangadu C, Olivares H, Shi L, Look D, Gallagher T. (2005) A SARS-CoV specific protein enhances virulence of an attenuated murine coronavirus. J Virol 79:11335-42. Pubmed Link
  2. Jia H, Look D, Hickey H, Shi L, Epping L, Netland J, Farzan M, Wohlford-Lenane C, Perlman S, McCray PB Jr. (2005) ACE2 receptor expression and SARS coronavirus infection depend on differentiation of human airway epithelia. JVirol 79:14614-21. Pubmed Link
  3. Perlman S, Dandekar A. (2005) Immunopathogenesis of coronavirus infections: Implications for SARS. Nature Reviews Immunology 5:917-27.Pubmed Link
  4. Butler N, Pewe L, Trandem K, Perlman S. (2006) Murine encephalitis caused by HCoV-OC43, a human coronavirus with broad species specificity, is partly immune-mediated. Virology 347:410-21. Pubmed Link
  5. Dandekar A, Perlman S. (2006) Severe acute respiratory syndrome (SARS). Encyclopedia of Life Sciences, London: Nature Publishing Group.http://www.els.net/doi:10.1038/npg.els.0004025
  6. Pewe L, Zhou H, Netland J, Tangadu C, Olivares H, Shi L, Look D, Gallagher T, Perlman S. (2006) A SARS-CoV-specific protein enhances virulence of an attenuated strain of mouse helpatitus virus, in The Nidoviruses: Towards Control of SARS and other Nidovirus Diseases, eds. Perlman S, Holmes KV, Adv Exp Med Biol 581:493-8.
  7. Jia HP, Look D, Hickey M, Shi L, Pewe L, Netland J, Farzan M, Wohlford-Lenane C, Perlman S, McCray PB Jr. (2006) Infection of human airway epithelia by SARS coronavirus is associated with ACE2 expression and localization, in The Nidoviruses: Towards Control of SARS and other Nidovirus Diseases, eds. Perlman S, Holmes KV, Adv Exp Med Biol 581:479-84.
  8. Butler N, Pewe L,Trandem K, Perlamn S. (2006) HCoV-OC43-induced encephalitis is in part immune-mediated, in The Nidoviruses: Towards Control of SARS and other Nidovirus Diseases, eds. Perlman S, Holmes KV, Adv Exp Med Biol 581:531-4.
  9. Tangudu C, Olivares H, Netland J, Perlman S, Gallagher T. (2007) SARS Coronavirus Protein 6 Accelerates Murine Coronavirus Infections. J Virol 81:1220-9. Pubmed Link
  10. McCray PB Jr, Pewe L, Wolford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia H, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look D, Perlman S. (2007) Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus. J Virol 81:813-21. Pubmed Link
  11. Netland J, Ferraro D, Pewe L, Olivares H, Gallagher T, Perlman S. (2007) Enhancement of murine coronavirus replication by SARS-CoV protein 6 requires the N-terminal hydrophobic region but not C-terminal sorting motifs. J Virol 81:11520-5. Pubmed Link
  12. Hussain S, Perlman S, Gallagher TM. (2008) Severe acute respiratory syndrome coronavirus protein 6 accelerates murine hepatitis virus infections by more than one mechanism. J Virol 82:7212-22. Pubmed Link
  13. DeDiego ML, Pewe L, Alvarez E, Rejas MT, Perlman S, Enjuanes L. (2008) Pathogenicity of severe acute respiratory coronavirus deletion mutants in HACE2 transgenic mice. Virology 376:379-89. Pubmed Link  
  14. Netland J, Meyerholz D, Moore S, Cassell M, Perlman S. (2008) SARS-CoV infection causes neuronal death in the absence of encephalitis in mice transgenic for human ACE2 mice. J Virol 82:7264-75. Pubmed Link  
  15. Nicholls J, Peiris M, Perlman S. (2008) Severe Acute Respiratory Syndrome: Epidemiology, pathogenesis and animals models. In Nidoviruses, Ed. S Perlman, T Gallagher, EJ Snijder, ASM Press, Washington, DC.
  16. Zhao J, Falcón A, Zhou H, Netland J, Enjuanes L, Pérez Breña P, Perlman S. (2009) Severe Acute Respiratory Syndrome-CoV protein 6 is required for optimal replication. J Virol 83:2368-73. Pubmed Link
  17. Perlman S, Netland J. (2009) Coronaviruses post-SARS: Update on replication and pathogenesis. Nature Reviews Microbiology 7:439-50.Pubmed Link
  18. Khanolkar A, Hartwig SM, Haag BA, Meyerholz DK, Harty JT, Varga SM. (2009) Toll-like receptor 4-deficiency increases disease and mortality after MHV-1 infection in susceptible C3H mice. J Virol 83:8946-56. Pubmed Link
  19. Khanolkar A, Hartwig SM, Haag BA, Meyerholz DK, Epping LL, Haring JS, Varga SM, Harty JT. (2009). Protective and pathologic roles of the immune response to mouse hepatitis virus type 1: implications for severe acute respiratory syndrome. J Virol 83:9258-72. Pubmed Link  
  20. Jia HP, Look DC, Tan P, Shi L, Hickey M, Gakhar L, Chappell MC, Wohlford-Lenane C, McCray PB Jr. (2009) Ectodomain shedding of angiotensin converting enzyme 2 in human airway epithelia, Am J Physiol Lung Cell Mol Physiol 297:L84-96. Pubmed Link  
  21. Wohlford-Lenane CL, Meyerholz DK, Perlman S, Zhou H, Tran D, Selsted ME, McCray PB Jr. (2009) Rhesus theta-defensin prevents death in a mouse model of severe acute respiratory syndrome coronavirus pulmonary disease. J Virol 83:11385-90. Pubmed Link
  22. Zhao J., Zhao J, Van Rooijen N, Perlman S. (2009) Evasion by stealth: inefficient immune activation underlies poor T cell response and severe disease in SARS-CoV-infected mice. PLoS Pathogens 5:e1000636. Pubmed Link
  23. Netland J, DeDiego ML, Zhao J, Fett C, Alvarez E, Nieto-Torres JL, Enjuanes L, Perlman S. (2010) Immunization with an attenuated severe acute respiratory syndrome coronavirus deleted in E protein protects against lethal respiratory disease. Virology 399:120-8. Pubmed Link
  24. O'Keefe BR, Giomarelli B, Barnard DL, Shenoy SR, Chan PK, McMahon JB, Palmer KE, Barnett BW, Meyerholz DK, Wohlford-Lenane CL, McCray PB Jr. (2010) Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae. J Virol 84:2511-21. Pubmed Link  
  25. Zhou H, Ferraro D, Zhao J, Hussain S, Shao J, Trujillo J, Netland J, Gallagher T, Perlman S. (2010) The N-terminal region of severe acute respiratory syndrome coronavirus protein 6 induces membrane rearrangement and enhances virus replication. J Virol 84:3542-51. Pubmed Link
  26. Khanolkar A, Fulton RB, Epping LL, Pham NL, Tifrea D, Varga SM, Harty JT. (2010) T cell epitope specificity and pathogenesis of mouse hepatitis virus-1-induced disease in susceptible and resistant hosts. J Immunol 185:1132-41. Pubmed Link
  27. Zhao J, Zhao J, Perlman S. (2010) T cell responses are required for protection from clinical disease and for virus clearance in severe acute respiratory syndrome coronavirus-infected mice. J Virol 84:9318-25.Pubmed Link  
  28. Hussain S, Gallagher T. (2010) SARS-coronavirus protein 6 conformations required to impede protein import into the nucleus. Virus Res 153:299-304.Pubmed Link
  29. Nieto-Torres JL, Dediego ML, Alvarez E, Jiménez-Guardeño JM, Regla-Nava JA, Llorente M, Kremer L, Shuo S, Enjuanes L. (2011) Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein. Virology. 415(2):69-82. Pubmed Link 
  30. Ahn DG, Lee W, Choi JK, Kim SJ, Plant EP, Almazán F, Taylor DR, Enjuanes L, Oh JW. (2011) Interference of ribosomal frameshifting by antisense peptide nucleic acids suppresses SARS coronavirus replication. Antiviral Res. 91(1):1-10.  Pubmed Link
  31. Shulla A, Heald-Sargent T, Subramanya G, Zhao J, Perlman S, Gallagher T. (2011) A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry. J Virol 85:873-82. Pubmed Link 
  32. DeDiego ML, Nieto-Torres JL, Jiménez-Guardeño JM, Regla-Nava JA, Alvarez E,  Oliveros JC, Zhao J, Fett C, Perlman S, Enjuanes L. (2011) Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress response and apoptosis. PLOS Pathogens 7:e1002315. Pubmed Link
  33. Adedeji AO, Singh K, Calcaterra NE, DeDiego ML, Enjuanes L, Weiss S, Sarafianos SG. (2012) Severe acute respiratory syndrome coronavirus replication inhibitor that interferes with the nucleic acid unwinding of the viral helicase.  Antimicrob Agents Chemother. 2012 Sep;56(9):4718-28.Pubmed Link
  34. Heald-Sargent T, Gallagher T. (2012) Ready, set, fuse!  The coronavirus spike protein and acquisition of fusion competence. Viruses. (4):557-80. Pubmed Link  
  35. Tanaka T, Kamitani W, DeDiego ML, Enjuanes L, Matsuura Y. (2012) Severe acute respiratory syndrome coronavirus nsp1 facilitates efficient propagation in cells through a specific translational shutoff of host mRNA. J Virol. 86(20):11128-37. Pubmed Link
  36. Mahlakõiv T, Ritz D, Mordstein M, DeDiego ML, Enjuanes L, Müller MA, Drosten C, Staeheli P. (2012) Combined action of type I and type III interferon restricts initial replication of severe acute respiratory syndrome coronavirus in the lung  but fails to inhibit systemic virus spread. J Gen Virol. 93(Pt 12):2601-5.  Pubmed Link
  37. Verdiá-Báguena C, Nieto-Torres JL, Alcaraz A, Dediego ML, Enjuanes L, Aguilella VM. (2013) Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge. Biochim Biophys Acta. 1828(9):2026-31. Pubmed Link
  38. Regla-Nava JA, Jimenez-Guardeño JM, Nieto-Torres JL, Gallagher TM, Enjuanes L, DeDiego ML. (2013) The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines. J Virol Methods. 193(2):639-46. Pubmed Link  
  39. Almazán F, DeDiego ML, Sola I, Zuñiga S, Nieto-Torres JL, Marquez-Jurado S, Andrés G, Enjuanes L. (2013) Engineering a replication-competent, propagation-defective  Middle East respiratory syndrome coronavirus as a vaccine candidate. MBio. 10;4(5). Pubmed Link
  40.  Zhao J, Zhao J, Legge K, Perlman S. (2011) Age-related increases in PGD(2) expression impair respiratory DC migration, resulting in diminished T cell responses upon respiratory virus infection in mice. J Clin Invest 121:4921-30. Pubmed Link
  41. Zhao J, Wohlford-Lenane C, Zhao J, Fleming E, Lane TE, McCray PB Jr, Perlman S. (2012) Intranasal Treatment with Poly(I•C) Protects Aged Mice from Lethal Respiratory Viral Infections. J Virol 86:11416-24. Pubmed Link
  42. Perlman S, Zhao J. (2013) Human coronavirus EMC is not the same as severe acute respiratory syndrome coronavirus. MBio. 4:pii:e00002-13.Pubmed Link
  43. Gallagher T, Perlman S. (2013) Public health: Broad reception for coronavirus. Nature 495:176-7. Pubmed Link
  44. de Groot RJ, Baker SC, Baric RS, Brown CS, Drosten C, Enjuanes L, Fouchier RA, Galiano M, Gorbalenya AE, Memish ZA, Perlman S, Poon LL, Sinjder EJ, Stephens GM, Woo PC, Zaki AM, Zambon M, Ziebuhr J. (2013) Middle East respiratory syndrome coronavirus (MERS-CoV): announcement of the Coronavirus Study Group. J Virol. 87:7790-2.Pubmed Link
  45. Perlman S, McCray PB. (2013) Person-to-person spread of the MERS coronavirus--an evolving picture. N Engl J Med. 369:466-7. Pubmed Link
  46. Fett C, DeDiego ML, Regla-Nava JA, Enjuanes L, Perlman S. (2013) Complete protection against severe acute respiratory syndrome coronavirus-mediated lethal respiratory disease in aged mice by immunization with a mouse-adapted virus lacking E protein. J Virol. 87:6551-9. Pubmed Link
  47. Perlman S. (2013) The middle East respiratory syndrome--how worried should we be? MBio. 4:e00531-13. Pubmed Link
  48. DeDiego ML, Nieto-Torres JL, Regla-Nava JA, Jimenez-Guardeño JM, Fernandez-Delgado R, Fett C, Castaño-Rodriguez C, Perlman S, Enjuanes L. (2014) Inhibition of NF-κB-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival. J Virol. 88:913-24. Pubmed Link
  49. Zhao J, Li K, Wohlford-Lenane C, Agnihothram SS, Fett C, Zhao J, Gale MJ, Baric RS, Enjuanes L, Gallagher T, McCray PB, Perlman S. (2014) Rapid generation of a mouse model for Middle East respiratory syndrome. Proc Natl Acad Sci U S A 111:4970-5. Pubmed Link
  50. Barlan A, Zhao J, Sarkar MK, Li K, McCray PB, Perlman S, Gallagher T. (2014) Receptor variation and susceptibility to Middle East respiratory syndrome coronavirus infection. J Virol. 88:4953-61. Pubmed Link
  51. Hemida M, Perera R, Al Jassim R, Kayali G, Siu L, Wang P, Chu K, Perlman S, Ali M, Alnaeem A, Guan Y, Poon L, Saif L, Peiris M. (2014) Seroepidemiology of Middle East respiratory syndrome (MERS) coronavirus in Saudi Arabia (1993) and Australia (2014) and characterisation of assay specificity. Euro Surveill. 19:pii: 20828. Pubmed Link
  52. Channappanavar R, Zhao J, Perlman S. (2014) T cell-mediated immune response to respiratory coronaviruses. Immunol Res. 59(1-3):118-28. Pubmed Link 
  53. Amazán F, Sola I, Zuñiga S, Marquez-Jurado S, Morales L, Becares M, Enjuanes L. (2014) Coronavirus reverse genetic systems: infectious clones and replicons. Virus Res.189:262-70. Pubmed Link
  54. Nieto-Torres JL, DeDiego ML, Verdiá-Báguena C, Jimenez-Guardeño JM, Regla-Nava JA, Fernandez-Delgado R, Castaño-Rodriguez C, Alcaraz A, Torres J, Aguilella VM, Enjuanes L. (2014) Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. PLoS Pathog.1;10(5) Pubmed Link
  55. Channappanavar R, Fett C, Zhao J, Meyerholz DK, Perlman S. (2014) Virus-specific memory CD8 T cells provide substantial protection from lethal severe acute respiratory syndrome coronavirus infection. J Virol. 88(19):11034-44.  Pubmed Link
  56.  Enjuanes L, Sola I, Frieman MB. (2014) Virus Research. Nidoviruses I. Foreword. Virus Res. 94:1-2. Pubmed Link
  57. Jimenez-Guardeño JM, Nieto-Torres JL, DeDiego ML, Regla-Nava JA, Fernandez-Delgado R, Castaño-Rodriguez C, Enjuanes L. (2014) The PDZ-binding motif of severe acute respiratory syndrome coronavirus envelope protein is a determinant of viral pathogenesis. PLoS Pathog. 10(8) Pubmed Link   
  58. DeDiego ML, Nieto-Torres JL, Regla-Nava JA, Jimenez-Guardeño JM, Fernandez-Delgado R, Fett C, Castaño-Rodriguez C, Perlman S, Enjuanes L. (2014) Inhibition of NF-κB-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival. J Virol. 88(2):913-24. Pubmed Link
  59. DeDiego ML, Nieto-Torres JL, Jimenez-Guardeño JM, Regla-Nava JA, Castaño-Rodriguez C, Fernandez-Delgado R, Usera F, Enjuanes L. (2014) Coronavirus virulence genes with main focus on SARS-CoV envelope gene. Virus Res. 194:124-37.  Pubmed Link
  60.  Fehr AR, Perlman S. (2015) Coronaviruses: an overview of their replication and pathogenesis. Methods Mol Biol. 1282:1-23. Pubmed Link
  61. Zhang N, Channappanavar R, Ma C, Wang L, Tang J, Garron T, Tao X, Tasneem S, Lu L, Tseng C, Zhou Y, Perlman S, Jiang S, Du L. (2015) Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus.  Cell Mol Immunology. Epub ahead of print Pubmed Link
  62. Fehr A, Athmer J, Channappanavar R, Phillips J, Meyerholz D, Perlman S.  The NSP3 macrodomain promotes virulence in mice with coronavirus-induced encephalitis.  J Virol.  2015 February. 89(3):1523-36. Pubmed Link 
  63. Zumla A, Perlman S, McNabb SJ, Shaikh A, Heymann DL, McCloskey B, Hui DS. (2015) Middle East respiratory syndrome in the shadow of Ebola. Lancet Respir Med. 3(2):100-2. Pubmed Link
  64. Almazán F, Márquez-Jurado S, Nogales A, Enjuanes L. (2015) Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes. Methods Mol Biol. 1282:135-52. doi: 10.1007/978-1-4939-2438-7_13. PubMed PMID: 25720478. Pubmed Link
  65. Jimenez-Guardeño JM, Regla-Nava JA, Nieto-Torres JL, DeDiego ML, Castaño-Rodriguez C, Fernandez-Delgado R, Perlman S, Enjuanes L. (2015) Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine. PLoS Pathog. 11(10). Pubmed Link
  66. Nieto-Torres JL, Verdiá-Báguena C, Jimenez-Guardeño JM, Regla-Nava JA, Castaño-Rodriguez C, Fernandez-Delgado R, Torres J, Aguilella VM, Enjuanes L. (2015) Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome. Virology. 485:330-9. Pubmed Link   
  67.  Regla-Nava J, Nieto-Torres J, Jimenez-Guardeño J, Fernandez-Delgado R, Fett C, Castaño-Rodriguez C, Perlman S, Enjuanes L, DeDiego M. (2015) Severe acute respiratory syndrome coronaviruses with mutations in the E protein are attenuated and promising vaccine candidates.  J Virol. 89(7):3870-87. Pubmed Link
  68. Zhao J, Perera R, Kayali G, Meyerholz D, Perlman S, Peiris M. (2015) Passive immunotherapy with dromedary immune serum in an experimental animal model for Middle East respiratory syndrome coronavirus infection.  J Virol. 89(11):6117-20.  Pubmed Link
  69. Mielech A, Deng X, Chen Y, Kindler E, Wheeler D, Mesecar A, Thiel V, Perlman S, Baker S. (2015) Murine coronavirus ubiquitin-like domain is important for papain-like protease stability and viral pathogenesis.  J Virol. 89(9):4907-17. Pubmed Link 
  70. Channappanavar R, Lu L, Xia S, Du L, Meyerholz D, Perlman S, Jiang S. (2015) Protective Effect of Intranasal Regimens Containing Peptidic Middle East Respiratory Syndrome Coronavirus Fusion Inhibitor Against MERS-CoV Infection.  J Infect Dis. 212(12):1894-903. Pubmed Link
  71. Hui D, Perlman S, Zumla A. (2015) Spread of MERS to South Korea and China.  Lancet Respir Med. 3(7):509-10. Pubmed Link
  72. Frieman M, Sola I, Enjuanes L. (2015) Virus Research. Foreword. Nidoviruses II. Virus Res. 16;202:1-2. Pubmed Link
  73. Petersen E, Hui D, Perlman S, Zumla A. (2015) Middle East Respiratory Syndrome - advancing the public health and research agenda on MERS - lessons from the South Korea outbreak.  Int J Infect Dis. 36:54-5.  Pubmed Link
  74. Perlman S. (2015) Research Driven by Curiosity: The Journey from Basic Molecular Biology and Virology to Studies of Human Pathogenic Coronaviruses.  PLoS Pathog. 11(7):e1005023. Pubmed Link
  75. Corti D, Zhao J, Pedotti M, Simonelli L, Agnihothram S, Fett C, Fernandez-Rodriguez B, Foglierini M, Agatic G, Vanzetta F, Gopal R, Langrish C, Barrett N, Sallusto F, Baric R, Varani L, Zambon M, Perlman S, Lanzavecchia A.  (2015) Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus.  Proc Natl Acad Sci U S A. 112(33):10473-8. Pubmed Link
  76. Vijay R, Hua X, Meyerholz D, Miki Y, Yamamoto K, Gelb M, Murakami M, Perlman S. (2015) Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.  J Exp Med. Epub. 212(11):1851-68. Pubmed Link
  77. Zumla A, Hui D, Perlman S. (2015) Middle East respiratory syndrome.  Lancet. 386(9997):995-1007.  Pubmed Link 
  78. Nieto-Torres JL, Verdiá-Báguena C, Castaño-Rodriguez C, Aguilella VM, Enjuanes L. (2015) Relevance of Viroporin Ion Channel Activity on Viral Replication and Pathogenesis. Viruses. 7(7):3552-73.  Pubmed Link
  79. Kusov Y, Tan J, Alvarez E, Enjuanes L, Hilgenfeld R. (2015) A G-quadruplex-binding macrodomain within the "SARS-unique domain" is essential for the activity of the  SARS-coronavirus replication-transcription complex. Virology. 484:313-22. Pubmed Link
  80. Li Y, Wan Y, Liu P, Zhao J, Lu G, Qi J, Wang Q, Lu X, Wu Y, Liu W, Zhang B, Yuen K, Perlman S, Gao G, Yan J. (2015) A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein.  Cell Res. 25(11):1237-49. Pubmed Link
  81. Earnest JT, Hantak MP, Park JE, Gallagher T. (2015) Coronavirus and influenza virus proteolytic priming takes place in tetraspanin-enriched membrane microdomains. J Virol. 89(11):6093-104.  Pubmed Link
  82. Li K, Wohlford-Lenane C, Perlman S, Zhao J, Jewell AK, Reznikov LR, Gibson-Corley KN, Meyerholz DK, McCray PB Jr. (2015) Middle East Respiratory Syndrome Coronavirus Causes Multiple Organ Damage and Lethal Disease in Mice Transgenic for Human Dipeptidyl Peptidase 4. J Infect Dis. Oct 20. pii: jiv499. Pubmed Link
  83. Meyerholz DK, Lambertz AM, McCray PB Jr. (2016) Dipeptidyl Peptidase 4 Distribution in the Human Respiratory Tract: Implications for the Middle East Respiratory Syndrome. Am J Pathol. pii: S0002-9440(15)00576-3.Pubmed Link